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western blot apparatus  (Bio-Rad)


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    Structured Review

    Bio-Rad western blot apparatus
    Western Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 44931 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/western+blot+apparatus/Protein+Standard+I/pmc12731447-81-25-27
    Average 99 stars, based on 44931 article reviews
    western blot apparatus - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    SDS Page:

    Article Title: Chloroform Extract from Fermented Viola mandshurica Regulates LPS-Induced Inflammation Response in RAW 264.7 Cells by Inhibiting iNOS and COX-2.
    Article Snippet: Introduction Inflammation is a normal physiological phenomenon that occurs in response to microbial infection, trauma, or injury such as heat and radiation [1].. Macrophages are well known as inflammatory cells that are important in the initiation of inflammatory responses.. They secrete many inflammatory mediators and inflammatory cytokines, playing an important role in the pathogenesis of inflammatory disease processes [2].

    Article Title: Regulation of Kv1.2 redox-sensitive gating by the transmembrane lectin LMAN2
    Article Snippet: Detection of LMAN2, LMAN1 and LMAN2L: Cells were lysed for 2hr at 4°C in NP-40 lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris-HCl) with 1% protease inhibitor cocktail (Sigma, P8430), 72 hours after transfection. .. Samples were separated on either 8% or 15% SDS-PAGE gels and transferred to nitrocellulose membranes using a Western blot apparatus (Bio-rad). ..

    Article Title: Activation of G-Protein-Coupled Estrogen Receptor 1 (GPER1) Reduces Progression of Vulvar Carcinoma Cells
    Article Snippet: Protein was diluted 1:1 with Laemmli buffer (Fresenius Kabi, Bad Homburg vor der Höhe, Germany), 5 mL LDS sample buffer (Thermo Fisher Scientific), and 1 mL NuPage sample-seducing agent (Thermo Fisher Scientific). .. The proteins were fractioned using SDS-PAGE and Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA). .. The membrane was incubated overnight at 4 °C with rabbit antihuman GAPDH at 1:1000 dilution (Cell Signaling Technology, Danvers, MA, USA), rabbit antihuman GPER1 at 1:2000 dilution (Thermo Fisher Scientific), rabbit antihuman ERα at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology, Dallas, TX, USA), or rabbit antihuman ERβ at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology).

    Article Title: Activation of G-Protein-Coupled Estrogen Receptor 1 (GPER1) Reduces Progression of Vulvar Carcinoma Cells.
    Article Snippet: 2023, 24, 13705 18 of 20 Germany), 5 mL LDS sample buffer (Thermo Fisher Scientific), and 1 mL NuPage sampleseducing agent (Thermo Fisher Scientific). .. The proteins were fractioned using SDS-PAGE and Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA). .. The membrane was incubated overnight at 4 ◦C with rabbit antihuman GAPDH at 1:1000 dilution (Cell Signaling Technology, Danvers, MA, USA), rabbit antihuman GPER1 at 1:2000 dilution (Thermo Fisher Scientific), rabbit antihuman ERα at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology, Dallas, TX, USA), or rabbit antihuman ERβ at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology).

    Article Title: Potato Protein Hydrolysate (PPH902) treatment in adipocytes inhibits lipogenesis by activating AMPK
    Article Snippet: Obesity is a chronic metabolic disease caused by unbalanced caloric intake, which has serious harm to health and increases the risk of hypertension, hyperlipidemia, diabetes and cardiovascular diseases.. Previous studies have shown that Alcalase treatment-derived potato protein hydrolysate (PPH) has the effects of regulating blood pressure and blood sugar in animal experiments, and has cardioprotective, hepato-protective activity and inhibited muscle protein degradation.. This study aims to screen the optimal conditions isolating potato protein hydrolysate with potential anti-obesity activity by regulating the mechanism of lipogenesis and lipolysis.

    Article Title: Potato protein hydrolysate (PPH902) exerts anti-lipogenesis and lipolysis-promoting effect by inhibiting adipogenesis in 3T3-L1 adipocytes.
    Article Snippet: Obesity is linked to cardiovascular disease, cerebrovascular disease, diabetes, and dyslipidemia, lowering quality of life, work productivity, and healthcare expenditures.. The aim of this present study is to investigate the mechanism of potato protein (PP) post-treatment in regulating lipogenesis and lipolysis in 3T3-L1 adipocytes.. 9% PP hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments.

    Membrane:

    Article Title: Chloroform Extract from Fermented Viola mandshurica Regulates LPS-Induced Inflammation Response in RAW 264.7 Cells by Inhibiting iNOS and COX-2.
    Article Snippet: Introduction Inflammation is a normal physiological phenomenon that occurs in response to microbial infection, trauma, or injury such as heat and radiation [1].. Macrophages are well known as inflammatory cells that are important in the initiation of inflammatory responses.. They secrete many inflammatory mediators and inflammatory cytokines, playing an important role in the pathogenesis of inflammatory disease processes [2].

    Article Title: Potato Protein Hydrolysate (PPH902) treatment in adipocytes inhibits lipogenesis by activating AMPK
    Article Snippet: Obesity is a chronic metabolic disease caused by unbalanced caloric intake, which has serious harm to health and increases the risk of hypertension, hyperlipidemia, diabetes and cardiovascular diseases.. Previous studies have shown that Alcalase treatment-derived potato protein hydrolysate (PPH) has the effects of regulating blood pressure and blood sugar in animal experiments, and has cardioprotective, hepato-protective activity and inhibited muscle protein degradation.. This study aims to screen the optimal conditions isolating potato protein hydrolysate with potential anti-obesity activity by regulating the mechanism of lipogenesis and lipolysis.

    Article Title: Potato protein hydrolysate (PPH902) exerts anti-lipogenesis and lipolysis-promoting effect by inhibiting adipogenesis in 3T3-L1 adipocytes.
    Article Snippet: Obesity is linked to cardiovascular disease, cerebrovascular disease, diabetes, and dyslipidemia, lowering quality of life, work productivity, and healthcare expenditures.. The aim of this present study is to investigate the mechanism of potato protein (PP) post-treatment in regulating lipogenesis and lipolysis in 3T3-L1 adipocytes.. 9% PP hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments.

    Western Blot:

    Article Title: Chloroform Extract from Fermented Viola mandshurica Regulates LPS-Induced Inflammation Response in RAW 264.7 Cells by Inhibiting iNOS and COX-2.
    Article Snippet: Introduction Inflammation is a normal physiological phenomenon that occurs in response to microbial infection, trauma, or injury such as heat and radiation [1].. Macrophages are well known as inflammatory cells that are important in the initiation of inflammatory responses.. They secrete many inflammatory mediators and inflammatory cytokines, playing an important role in the pathogenesis of inflammatory disease processes [2].

    Article Title: Effects of PCNA Stability on the Formation of Mutations
    Article Snippet: .. Gels were run for 90 min in 140 V in a Bio-Rad Western blot apparatus (Hercules, CA, USA). .. Afterwards, the gel was taken apart and proteins were transferred using the Bio-Rad transfer apparatus for 90 min in 400 mA to cellulose membranes.

    Article Title: Regulation of Kv1.2 redox-sensitive gating by the transmembrane lectin LMAN2
    Article Snippet: Detection of LMAN2, LMAN1 and LMAN2L: Cells were lysed for 2hr at 4°C in NP-40 lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris-HCl) with 1% protease inhibitor cocktail (Sigma, P8430), 72 hours after transfection. .. Samples were separated on either 8% or 15% SDS-PAGE gels and transferred to nitrocellulose membranes using a Western blot apparatus (Bio-rad). ..

    Article Title: Activation of G-Protein-Coupled Estrogen Receptor 1 (GPER1) Reduces Progression of Vulvar Carcinoma Cells
    Article Snippet: Protein was diluted 1:1 with Laemmli buffer (Fresenius Kabi, Bad Homburg vor der Höhe, Germany), 5 mL LDS sample buffer (Thermo Fisher Scientific), and 1 mL NuPage sample-seducing agent (Thermo Fisher Scientific). .. The proteins were fractioned using SDS-PAGE and Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA). .. The membrane was incubated overnight at 4 °C with rabbit antihuman GAPDH at 1:1000 dilution (Cell Signaling Technology, Danvers, MA, USA), rabbit antihuman GPER1 at 1:2000 dilution (Thermo Fisher Scientific), rabbit antihuman ERα at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology, Dallas, TX, USA), or rabbit antihuman ERβ at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology).

    Article Title: Activation of G-Protein-Coupled Estrogen Receptor 1 (GPER1) Reduces Progression of Vulvar Carcinoma Cells.
    Article Snippet: 2023, 24, 13705 18 of 20 Germany), 5 mL LDS sample buffer (Thermo Fisher Scientific), and 1 mL NuPage sampleseducing agent (Thermo Fisher Scientific). .. The proteins were fractioned using SDS-PAGE and Western blot apparatus (Bio-Rad Laboratories, Hercules, CA, USA). .. The membrane was incubated overnight at 4 ◦C with rabbit antihuman GAPDH at 1:1000 dilution (Cell Signaling Technology, Danvers, MA, USA), rabbit antihuman GPER1 at 1:2000 dilution (Thermo Fisher Scientific), rabbit antihuman ERα at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology, Dallas, TX, USA), or rabbit antihuman ERβ at 1:200 dilution from a 100 μg/mL stock solution (Santa Cruz Technology).

    Article Title: Potato Protein Hydrolysate (PPH902) treatment in adipocytes inhibits lipogenesis by activating AMPK
    Article Snippet: Obesity is a chronic metabolic disease caused by unbalanced caloric intake, which has serious harm to health and increases the risk of hypertension, hyperlipidemia, diabetes and cardiovascular diseases.. Previous studies have shown that Alcalase treatment-derived potato protein hydrolysate (PPH) has the effects of regulating blood pressure and blood sugar in animal experiments, and has cardioprotective, hepato-protective activity and inhibited muscle protein degradation.. This study aims to screen the optimal conditions isolating potato protein hydrolysate with potential anti-obesity activity by regulating the mechanism of lipogenesis and lipolysis.

    Article Title: Potato protein hydrolysate (PPH902) exerts anti-lipogenesis and lipolysis-promoting effect by inhibiting adipogenesis in 3T3-L1 adipocytes.
    Article Snippet: Obesity is linked to cardiovascular disease, cerebrovascular disease, diabetes, and dyslipidemia, lowering quality of life, work productivity, and healthcare expenditures.. The aim of this present study is to investigate the mechanism of potato protein (PP) post-treatment in regulating lipogenesis and lipolysis in 3T3-L1 adipocytes.. 9% PP hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments.

    Article Title: The Cell Polarity Protein Scribble Is Involved in Maintaining the Structure of Neuromuscular Junctions, the Expression of Myosin Heavy Chain Genes, and Endocytic Recycling in Adult Skeletal Muscle Fibers
    Article Snippet: .. Electrophoretic migration was conducted at 120V for approximately 4 h. Protein transfer to nitrocellulose membranes was carried out for 2 h at 2.5A using a Western-Blot apparatus (Bio-Rad, Feldkirchen, Germany). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Potato Protein Hydrolysate (PPH902) treatment in adipocytes inhibits lipogenesis by activating AMPK
    Article Snippet: Obesity is a chronic metabolic disease caused by unbalanced caloric intake, which has serious harm to health and increases the risk of hypertension, hyperlipidemia, diabetes and cardiovascular diseases.. Previous studies have shown that Alcalase treatment-derived potato protein hydrolysate (PPH) has the effects of regulating blood pressure and blood sugar in animal experiments, and has cardioprotective, hepato-protective activity and inhibited muscle protein degradation.. This study aims to screen the optimal conditions isolating potato protein hydrolysate with potential anti-obesity activity by regulating the mechanism of lipogenesis and lipolysis.

    Nucleic Acid Electrophoresis:

    Article Title: Potato protein hydrolysate (PPH902) exerts anti-lipogenesis and lipolysis-promoting effect by inhibiting adipogenesis in 3T3-L1 adipocytes.
    Article Snippet: Obesity is linked to cardiovascular disease, cerebrovascular disease, diabetes, and dyslipidemia, lowering quality of life, work productivity, and healthcare expenditures.. The aim of this present study is to investigate the mechanism of potato protein (PP) post-treatment in regulating lipogenesis and lipolysis in 3T3-L1 adipocytes.. 9% PP hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments.

    Migration:

    Article Title: The Cell Polarity Protein Scribble Is Involved in Maintaining the Structure of Neuromuscular Junctions, the Expression of Myosin Heavy Chain Genes, and Endocytic Recycling in Adult Skeletal Muscle Fibers
    Article Snippet: .. Electrophoretic migration was conducted at 120V for approximately 4 h. Protein transfer to nitrocellulose membranes was carried out for 2 h at 2.5A using a Western-Blot apparatus (Bio-Rad, Feldkirchen, Germany). ..



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